"A casa do jardim"

A apresentação de uma intervenção de escala e intenção doméstica como testemunho de uma possibilidade serena de diálogo do valor do antigo com novos sentidos de habitar e construir

Perfusion Cell Seeding and Expansion in Dual Mechanical Stimulation Bioreactor for In Vitro Tissue Development

BACKGROUND: Engineered tissues are an exciting potential source of small diameter vascular grafts due to limited supply and patency of available alternatives. Engineered tissue vascular grafts (ETVGs) will provide physiological function that resembles native arteries and maintain the required mechanical properties as they integrate with host tissue. Mechanical stimulation during incubation encourages proper cell alignment and increases extracellular matrix deposition. The enhanced organization of the engineered tissue leads to improved compliance over synthetic alternatives without sacrificing strength and may lead to better integration in vivo. METHODS: We have developed a bioreactor that mechanically trains grafts during incubation. To test the seeding efficiency of the bioreactor, rat vascular smooth muscle cells (VSMC) were seeded onto electrospun PCL scaffolds by perfusion at various cell concentrations then incubated Page | 15 for 1 week under static conditions. We assessed gross morphology with H&E; collagen with picrosirius red; and VSMC density with DAPI. ETVGs were further evaluated with mechanical testing and scanning electron microscopy to evaluate mechanical and microstructural properties. RESULTS: Cells were successfully seeded evenly onto the luminal surface of electrospun PCL scaffolds. Cells remained viable and continued to proliferate and deposit ECM throughout incubation. CONCLUSIONS: Progress in the ETVG paradigm requires a systematic approach toward better understanding of the cause-effect interplay between implant properties, host reactions, and their modulation with controllable parameters. Future directions involve the assessment of the effects of mechanical training on growth and remodeling of engineered tissues in vitro and subsequent effects on the foreign body response post-implantation in a murine model.https://scholarscompass.vcu.edu/gradposters/1115/thumbnail.jp

PERANCANGAN SUDU KINCIR AIR TIPE UNDERSHOT BERBASIS MATERIAL ACRYLIC

Dalam perancangann sudu kincir tipe1 undershot ini mengunakan material berbahan acrylic dikarenakan1massa jenis acrilik (massa1jenis acrylic 1,18 g/ jelas lebih ringan dibandingkan dengan massa jenis besi yang di gunakan kincir air pada umumnya, maka jika bahan sebuah sudu pada kincir air tersebut lebih ringan diharapkan putaran pada kincir air lebih kencang dan menghasilkan nilai torsi yang besar sehingga dapat menciptakan arus listrik yang maksimal. Dan dalam peneltian ini penulis menggunakan 3 variasi sudu yaitu sudu 18, sudu 14 dan sudu 8 dan akan uji sebanyak 12 kali. Dengan beban sebesar 8 lb serta memeliki 2 variasi kemiringan aliran sungai buatan yaitu 24 cm dan 14 cm guna untuk mengetahui kecepatan putaran turbin, tegangan listrik, ampere, kecepatan aliran dan efisiensi dari setiap sudu yang diuj

Elastic Foundations as Heterogeneous Adventitial Boundary Condition for the Assessment of Aortic Wall and Peri-Aortic Stiffness from Dense-MRI Data Using Inverse FEM Approach

Background: The establishment of in vivo, patient-specific, and regionally resolved techniques to quantify aortic properties is key for improving risk assessment in clinical practice and scientific understanding of cardiovascular growth and remodeling. Many in vivo studies quantify vascular stiffness using Pulse Wave Velocity. This method provides an averaged measure of stiffness for the entire aorta, ignoring variations in wall stiffness and boundary conditions. Previous studies using Displacement Encoding with Stimulated Echoes Magnetic Resonance Imaging (DENSE-MRI) suggested that the infrarenal abdominal aorta (IAA) deforms heterogeneously throughout the cardiac cycle. Method: Herein, we hypothesize that the aortic wall strain heterogeneity is driven in healthy aortas by adventitial tethering to perivascular tissues that can be modeled with elastic foundation boundary conditions (EFBC) using a collection of linear-springs with a circumferential distribution of stiffness. Nine healthy-human IAAs were modeled using patient-specific imaging and displacement fields from SSFP and DENSE MRI, followed by assessment of aortic wall properties and heterogeneous EFBC parameters using inverse Finite Element Method (FEM). Results: In contrast to traction-free boundary condition, prescription of EFBC reduced the nodal displacement error by 60% and reproduced the DENSE-derived strain distribution. Estimated aortic stiffness was in agreement with previously reported experimental test data. The distribution of normalized EFBC stiffness was consistent among all patients and spatially correlated to standard peri-aortic anatomical features. Conclusion: Results suggest that EFBCs can be generalized for human adults with normal anatomy. This approach is computationally inexpensive, making it ideal for large-population clinical research and incorporation into computational cardiovascular fluid-structure analyses.https://scholarscompass.vcu.edu/gradposters/1113/thumbnail.jp

The Museum of the Void

Reflexão sobre as possbilidades de significação e intervenção sobre os enormes espaços vazios gerados pela desactivção de sistemas de silagem de cereais. O potencial programático que o próprio espaço vazio pode, em si, encerrar, é ponto de partida para considerações arquitectónicas, onde se convoca também o valor da proposta projectual como instrumento de ensaio.Trata-se de um texto que integra o catálogo da Exposição homónima, a partir de exploração projectual em domínio académico avançado (Mestrado Integrado)